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In vitro pharmacological approaches to the study of G protein-coupled receptors: radioligand binding, autoradiography, 2nd messenger assays (cAMP, cGMP, inositol phosphates) intracellular Ca2+ measurements, immunohistochemistry, reporter gene assays, Western blotting, primary cell culture. confocal microscopy. Classical pharmacological preparations: isolated blood vessels and other smooth muscle preparations.
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