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职业迁徙
个人简介
Thanks to my background in physics, I developed during my PhD an experimental setup that is able to switch the stiffness of the mechanical environment of a single cell in real time and at the same time to quantify the cellular deformation and the force generated by the cell. At that time I was under the supervision of Dr. Atef Asnacios (a physicist) at the Matière et ystèmes Complexes laboratory, University Paris7 (Paris, France). I benefited from a dynamic interdisciplinary environment that expanded my knowledge and my experimental skills in the fields of fundamental physical measurements, quantitative biology and cell mechano-sensibility, which at the time was strongly emerging. My first efforts in cell biology were fruitful (Mitrossilis et al. 2009) as I found that the actin and myosin complex acts as a sensor of the mechanical environment. This greatly enhanced my enthusiasm for science, fostering my curiosity to understand single cell mechanosensing. The need to study the dynamics of cellular functions was rising, and I thus started to formulate new hypotheses and plan experiments regarding the influence of external mechanical properties on cell behavior. In order to be able to answer this question, I had to update the experimental setup, the traction force measurement device, and to be innovative in fundamental physical metrology (Mitrossilis et al., European Patent EP09305991, 2009). This gave rise to fundamental answers in the field of cell mechanosensing (Mitrossilis et al., 2010). I started to collaborate with Jocelyn Etienne, with whom, based on my experimental data, we developed a theoretical model of cell sensing (Etienne et al 2015).
My interests at the end of my PhD were already diverging from the lab’s : I wanted to understand how mechanical forces interact with cell biochemistry during embryo development in a physiological context. I thus decided to join Dr. Emmanuel Farge’s lab at the Institut Curie (Paris, France) since I wanted to expand my knowledge in genetics while using my expertise in mechanics, in order to develop an innovative technique that allows to mimic physiological apical deformations of single cells of the mesoderm of the fruit fly embryo, Drosophila melanogaster. The first task was to be able to magnetize tissue in a biocompatible way, and the second task to generate controlled physiological mechanical stimuli at the cell scale inside a developing embryo and look at the dynamics of cell response. To do so, I collaborated with Christine Ménager to produce biocompatible vesicles containing magnetic particles, and with Nora Dempsey for the design and characterization of micromagnets. Then, combining in real time spinning- disc microscopy, genetics, image quantification and advanced magnetic stimulation, I discovered that apical cell deformation generates a mechanical force that activates the first morphogenetic event, gastrulation in Drosophila (Mitrossilis et al. 2017, Brunet et al. 2013, Roëper et al 2018).
研究兴趣
论文共 10 篇作者统计合作学者相似作者
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Thanh Huong Nguyen Ho-Bouldoires, Kévin Sollier, Laura Zamfirov,Florence Broders-Bondon,Démosthène Mitrossilis, Sebastian Bermeo,Coralie L. Guerin,Anna Chipont,Gabriel Champenois,Renaud Leclère,Nicolas André,Laurent Ranno,
crossref(2018)
作者统计
#Papers: 10
#Citation: 810
H-Index: 7
G-Index: 10
Sociability: 4
Diversity: 0
Activity: 0
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