基本信息
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Career Trajectory
Bio
The goal of our research is to develop new methods and instrumentation for the structural characterization of proteins and their post-translational modifications at the low femtomole/attomole level and to apply these new methods to important structural problems in cell biology and immunology. Towards this end, we have pioneered the use of nanoflow HPLC in conjunction with microelectrospray ionization on ion trap and Fourier transform mass spectrometers. Briefly stated, the approach involves the use of proteolytic enzymes to convert the protein or group of proteins into a complex mixture of peptides, which are then fractionated by nanoflow-HPLC and eluted directly into the mass spectrometer. Mixtures containing thousands of different peptides can be analyzed in this manner. Protonated peptides of a particular mass are selected under computer control of the instrument, fragmented on collision with helium atoms and the resulting fragments are then separated and mass analyzed.
Research Interests
Papers共 681 篇Author StatisticsCo-AuthorSimilar Experts
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iScienceno. 4 (2024): 109458-109458
Melissa R. Leyden,Brent Gowen,Rodrigo Gonzalez-Romero,Jose Maria Eirin-Lopez,Bo-Hyun Kim,Fumio Hayashi, Jay McCartney, Patrick C. Zhang,Miyoko Kubo-Irie,Jeffrey Shabanowitz,Donald F. Hunt,Patrick Ferree,
Biochemistry and Cell Biologyno. 3 (2024): 238-251
Joey J. Kelly,Nathaniel Bloodworth, Qianqian Shao, Jeffery Shabanowitz,Donald Hunt,Jens Meiler,Marcos M. Pires
biorxiv(2023)
bioRxiv : the preprint server for biology (2023)
Epigeneticsno. 1 (2023): 2276425
Circulation Research (2023)
bioRxiv : the preprint server for biology (2023)
iScienceno. 9 (2021): 102971-102971
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