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个人简介
Research activity:
Basic Medicine and Biomedicine – Study of molecular and cellular mechanisms associated with human pathologies:
1. Lysosomal storage diseases: study of immune system alterations in Mucopolysaccharidosis II and VI patients (2014–2015)
2. Alzheimer's disease: study of ABC transporters as amyloid-β clearance mechanisms in Alzheimer's disease mouse model (2017)
3. Aging: study of protein aggregation across lifespan in mice (2017–2018)
4. Nuclear envelopathies: study of LAP1's physiological role and contribution of its dysfunction to the development of human diseases (2017–Present)
Technical skills:
1. Cell culture and other related techniques: isolation of human peripheral blood mononuclear cells; production of Epstein-Barr virus (EBV) and establishment of EBV-transformed human B cell lines; mammalian cell culture (human fibroblasts, SH-SY5Y and HeLa cells); cell viability assays; transfection of mammalian cell lines
2. Cell biology: immunocytochemistry; fluorescence-activated cell sorting (FACS)
3. Molecular biology: bacterial transformation; plasmid DNA amplification and purification; genomic DNA isolation
4. Biochemistry: extraction and quantification of total protein in mammalian cell lines and mouse tissues; isolation of detergent-insoluble and soluble protein fractions; protein co-immunoprecipitation; subcellular fractionation of proteins; blot overlay assay; SDS-PAGE; immunoblotting; enzyme-linked immunosorbent assay (ELISA); agarose gel electrophoresis
5. Microscopy: epifluorescence and confocal microscopy
6. Bioinformatics: FlowJo, GraphPad Prism, Image Lab, ImageJ/Fiji, STRING, PANTHER, Cytoscape
研究兴趣
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Tissue-Specific Cell Signalingpp.1-32, (2020)
AGING CELLno. 5 (2020): e13143-e13143
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