基本信息
浏览量:34
![](https://originalfileserver.aminer.cn/sys/aminer/icon/show-trajectory.png)
个人简介
24/10/2018 to present: Assistant Researcher, University of Algarve/Centre of Marine Sciences (CCMAR); 01/09/2017 to 28/02/2018: Invited Assistant Professor, University of Algarve: Techniques in Molecular and Cellular Biology, Master classes. Department of Biological Sciences and Bioengineering; 01/10/2014 to 30/09/2017: Postdoc researcher at the Functional Biochemistry and Proteomics (FBP) research group, under FCT fellowship SFRH/BPD/70277/2010, Unveiling molecular mechanisms of GRP as calcification inhibitor associated with chronic kidney disease (CKD) vascular calcification. CCMAR; 01/10/2011 to 30/9/2014: Postdoc researcher at the FBP research group, under FCT fellowship SFRH/BPD/70277/2010, Studies for the establishment of GRP as a biomarker for ectopic calcifications. Insights into the role of GRP in tumoral processes. CCMAR; 01/10/2011 to 30-9-2011: Postdoc researcher at the FBP research group, under FCT fellowship SFRH/BPD/70277/2010, 1)Studies for the establishment of GRP as a biomarker for ectopic calcifications. Insights into the role of GRP in tumoral processes. 2) Unveiling molecular mechanisms of GRP as calcification inhibitor associated with chronic kidney disease (CKD) vascular calcification. CCMAR/University of Algarve.
14-1-2011 a 30-9-2011- PostDoc researcher, under the FCT project PTDC/SAU-ESA/101186/2008, at the recently created CCMAR research group, the Functional Biochemistry and Proteomics (FBP) research group headed by Prof Dina Simes. CCMAR/ University of Algarve
2005-2010- PhD student at EDGE/CCMAR- University of Algarve
1999- Degree in Biochemistry- University of Algarve
Scientific area: Proteomics and molecular and cellular biology applied to the study of Vitamin K dependent (VKD) proteins that are associated with physiological and pathological tissue mineralization (vascular, skin and skeleton). Since the discovery of GRP during my PhD thesis I’m focused on studies to unveil GRP function and molecular mechanism underlying its function, the relation of GRP with calcification-related inflammatory diseases, and the establishment of GRP as a biomarker for pathological calcification and inflammatory processes, with the development and validation of specific bioassays to measure serum levels and gamma-carboxylation status of the protein, suitable for research and medical applications.
Specialization Domain:Molecular and Cellular Biology of mineralized tissues, ectopic mineralization pathologies of soft tissues, and bone and cartilage biology, with experience in a broad range of techniques used in this area, such as purification of nucleic acids, cloning and sequencing of cDNAs and genes, expression analysis by Northen blot and Real time PCR, construction and screening of cDNA and genomic libraries, identification of sites of gene expression, by in situ hybridization, and protein accumulation by immunohistochemistry, gene expression analysis through functional analysis of promoters, development and maintenance of cell cultures, among others. Protein purification and biochemical characterization of VKD proteins from vertebrates, using several methods for protein analysis, such as SDS-PAGE, western and dot blotting, specific gel staining, and transfer to PVDF membranes for sequence analysis. In vitro production and characterization of recombinant proteins produced in E. coli by mammalian cell systems.
Current research interests: Understanding the molecular mechanisms involved in the onset and development of highly prevalent calcification related chronic inflammatory diseases as cardiovascular disease, and osteoarthritis. Ectopic mineralization and inflammation related pathologies of the cardiovascular system, cartilage, skin and soft tissues. Understanding the function of the vitamin K-dependent protein Gla-rich protein (GRP) in Humans and its relevance in disease states. Discovery of novel biomarkers and biological innovative tools for application in diagnostics and therapeutics, such as the development of GRP-based bioassays for medical application in diagnostic and patient monitoring. Establishment of cell in-vitro and ex-vivo model systems suitable for functional studies and translation for human in vivo situation.
研究兴趣
论文共 5 篇作者统计合作学者相似作者
按年份排序按引用量排序主题筛选期刊级别筛选合作者筛选合作机构筛选
时间
引用量
主题
期刊级别
合作者
合作机构
作者统计
#Papers: 64
#Citation: 1573
H-Index: 23
G-Index: 39
Sociability: 5
Diversity: 3
Activity: 31
合作学者
合作机构
D-Core
- 合作者
- 学生
- 导师
数据免责声明
页面数据均来自互联网公开来源、合作出版商和通过AI技术自动分析结果,我们不对页面数据的有效性、准确性、正确性、可靠性、完整性和及时性做出任何承诺和保证。若有疑问,可以通过电子邮件方式联系我们:report@aminer.cn