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Research Description
My laboratory studies how nuclear transport and signal transduction control the compartmentalization and activity of transcription factors, particularly in the context of prostate cancer. A major focus of these studies is the androgen receptor (AR), a steroid hormone receptor that is critical for prostate cell growth. Translocation of AR through the nuclear pore complex (NPC) relies on features common to most nuclear transport pathways: (i) the use of nuclear import or export signals; (ii) the recognition of these signals by receptors that mediate translocation through the NPC; and (iii) RanGTPase-dependent assembly and disassembly of transport complexes.
The signals that specify nuclear import and export of AR, the receptors that mediate AR translocation, and the role of the RanGTPase in AR transport are all under investigation in my laboratory. Nuclear export would be expected to provide an effective mechanism for terminating the transcriptional response to androgen, however, we have recently found that AR translocation to the cytoplasm is important for its activity in the nucleus. This apparent paradox may reflect an undefined step in AR maturation, or crosstalk between AR and signal transduction pathways in the cytoplasm. AR is the target of multiple kinases, and we have generated phosphosite antibodies to study the pathways and functions of phospho-regulation. In the course of these studies we discovered a novel mechanism for loading protein phosphatase 2A (PP2A) onto AR. The loading mechanism requires small t antigen, a product encoded by SV40 that binds and alters the structure of a PP2A subunit. Current experiments are aimed at defining the structural basis of the PP2A loading reaction, as well as determining the cellular factors that mediate PP2A loading onto AR in untransformed cells.
While biochemical analysis is the cornerstone of our nuclear transport and signal transduction studies, we also employ cell biological approaches including microinjection, fluorescence microscopy and real-time imaging in live cells, and animal models of tumorigenesis. Our studies benefit from ongoing collaborations with groups that specialize in mass spectrometry, pathology, and prostate cancer.
My laboratory studies how nuclear transport and signal transduction control the compartmentalization and activity of transcription factors, particularly in the context of prostate cancer. A major focus of these studies is the androgen receptor (AR), a steroid hormone receptor that is critical for prostate cell growth. Translocation of AR through the nuclear pore complex (NPC) relies on features common to most nuclear transport pathways: (i) the use of nuclear import or export signals; (ii) the recognition of these signals by receptors that mediate translocation through the NPC; and (iii) RanGTPase-dependent assembly and disassembly of transport complexes.
The signals that specify nuclear import and export of AR, the receptors that mediate AR translocation, and the role of the RanGTPase in AR transport are all under investigation in my laboratory. Nuclear export would be expected to provide an effective mechanism for terminating the transcriptional response to androgen, however, we have recently found that AR translocation to the cytoplasm is important for its activity in the nucleus. This apparent paradox may reflect an undefined step in AR maturation, or crosstalk between AR and signal transduction pathways in the cytoplasm. AR is the target of multiple kinases, and we have generated phosphosite antibodies to study the pathways and functions of phospho-regulation. In the course of these studies we discovered a novel mechanism for loading protein phosphatase 2A (PP2A) onto AR. The loading mechanism requires small t antigen, a product encoded by SV40 that binds and alters the structure of a PP2A subunit. Current experiments are aimed at defining the structural basis of the PP2A loading reaction, as well as determining the cellular factors that mediate PP2A loading onto AR in untransformed cells.
While biochemical analysis is the cornerstone of our nuclear transport and signal transduction studies, we also employ cell biological approaches including microinjection, fluorescence microscopy and real-time imaging in live cells, and animal models of tumorigenesis. Our studies benefit from ongoing collaborations with groups that specialize in mass spectrometry, pathology, and prostate cancer.
研究兴趣
论文共 155 篇作者统计合作学者相似作者
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ACS chemical biologyno. 11 (2023): 2377-2384
Chunsong Yang,Krzysztof Wierbiłowicz,Natalia M Dworak,Song Yi Bae, Sachi B. Tengse, Nicki Abianeh,Justin M. Drake,Tarek Abbas,Aakrosh Ratan,David Wotton,Bryce M Paschal
Cancer Research Communicationsno. 4 (2023): 592-606
Chunsong Yang,Krzysztof Wierbilowicz,Natalia M Dworak,Song Yi Bae, Sachi B Tengse, Nicki Abianeh,Justin M Drake,Tarek Abbas,Aakrosh Ratan,David Wotton,Bryce M Paschal
crossref(2023)
Encyclopedia of Biological Chemistry IIIpp.388-392, (2021)
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作者统计
#Papers: 155
#Citation: 12002
H-Index: 56
G-Index: 56
Sociability: 6
Diversity: 3
Activity: 18
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