Bruno Guigliarelli

Bruno GUIGLIARELLI Present position: Professor in Physics at Aix-Marseille University, France (Exceptional Class Professor PR-CE2). - Head of the group of “Biophysics of Metalloproteins (20 researchers, including 13 permanent positions) in the Lab of Bioenergetics and Protein Engineering (BIP-UMR 7281), CNRS, Marseille, France. - Responsible of the Aix-Marseille EPR Platform, south site of the French EPR network (INFRANALYTICS-CNRS), and of the EPR-MRS site in the European MOSBRI network. Education Ecole Normale Supérieure - Cachan (1979-85); Aggregation in Physical Chemistry (1982) PhD in Chemical-Physics (1986); Habilitation à Diriger des Recherches (1990) at University of Aix-Marseille I. Professional experience 1987-93: CNRS researcher, Lab of Electronics of Condensed Matter (LEMCO-URA 784, Marseille) 1992-1994: Visiting researcher, R.C. Cammack Lab, King’s College, London, UK 1995-99: CNRS researcher Lab of Bioenergetics and Protein Engineering (BIP-UPR9036, Marseille) Since 1999: Professor in Physics at Aix-Marseille University (section 28), (BIP-UMR7281, Marseille) Other responsibilities: 2002-present: Head of the group of “Biophysics of Metalloproteins (BIP-UMR7281) 2006-2012: Director Lab of Bioenergetics and Protein Engineering (BIP-UPR9036, 80 people) 2013-2018: President of the French Group of Magnetism and Magnetic Resonances (MRM-French Chemical Society, SCF-SFP). 2007-2011: President of the French EPR Group (ARPE) 2004-2018: Responsible of the Master in Physics (Aix-Marseille University, 150 students, 8 master specialties) 2002-present: Coordinator of the Aix-Marseille EPR platform. 2010-2022: Coordinator of about 12 research projects including: Région MocoCAT (2012-14, 288 k€) MicroBioCO2 (2021-2024, 165 k€), ANR Spinfold (2009-14, 620 k€), ANR Molyère (2016-21, 590 k€) Research field: Structural and functional properties of complex enzymes involved in bioenergetic metabolisms of prokaryotes. Approaches based on advanced EPR techniques (ENDOR, ELDOR, ESEEM, HYSCORE), optical and magneto-optical spectroscopies, redox potentiometry, theoretical calculations. Magnetic and structural properties of metal cofactors and radical intermediates in biological systems. Dynamics and structural properties of flexible proteins and supramolecular assembly studied by spin labeling coupled to EPR. Development of new EPR probes. 173 publications in international journals. (>6180 citations, « h » = 49) – Supervision of 13 PhD Thesis. Selection of 5 recent publications: 1 - Abou Hamdan A, Burlat B, Gutiérrez-Sanz O, Liebgott PP, Baffert C, De Lacey AL, Rousset M, Guigliarelli B, Léger C, Dementin S. O(2)-independent formation of the inactive states of NiFe hydrogenase. Nat Chem Biol. 2013 9(1):15-7 2- Rendon J, Biaso F, Ceccaldi P, Toci R, Seduk F, Magalon A, Guigliarelli B, Grimaldi S. Elucidating the Structures of the Low- and High-pH Mo(V) Species in Respiratory Nitrate Reductase: A Combined EPR, 14,15N HYSCORE, and DFT Study. Inorg Chem. 2017, 56 (8), 4423-4435. 3-- Karthikeyan G, Bonucci A, Casano G, Gerbaud G, Abel S, Thomé V, Kodjabachian L, Magalon A, Guigliarelli B, Belle V, Ouari O, Mileo E. A Bioresistant Nitroxide Spin Label for In-Cell EPR Spectroscopy: In Vitro and In Oocytes Protein Structural Dynamics Studies. Angew Chem Int Ed Engl. 2018 Jan 26; 57(5):1366-1370. 4- Zeamari K, Gerbaud G, Grosse S, Fourmond V, Chaspoul F, Biaso F, Arnoux P, Sabaty M, Pignol D, Guigliarelli B, Burlat B. Tuning the redox properties of a [4Fe-4S] center to modulate the activity of Mo-bisPGD periplasmic nitrate reductase. Biochim Biophys Act- Bioenerg. 2019, 1860(5), 402-413. 5- Al-Attar S., Rendon J., Sidore M., Duneau JP., Seduk F., Biaso F., Grimaldi S., Guigliarelli B., Magalon M. Gating of substrate access and long-range proton transfer in Escherichia coli nitrate reductase A: the essential role of a remote glutamate residue" ACS Catal 2021, 11, 23, 14303-14318, DOI: 10.1021/acscatal.1c03988