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The ensemble of molecular chaperones are central components of the cellular machinery that establishes and maintains protein homeostasis. Chaperones assist native folding of newly synthesized proteins and repair and eliminate misfolded and aggregated proteins, and therefore have fundamental impact on cell physiology, aging and disease. The goal of our research is to understand the intricate functional network of chaperones and its interplay with proteases in protein biogenesis and quality control, and the molecular working principles of chaperone machines. Furthermore, we want to elucidate causes and consequences of protein aggregation related to disease, in particular neurodegeneration and cancer. As models we are using E. coli, S. cerevisiae, C. elegans and human cells, and we are employing multi-disciplinary approaches ranging from genetics and molecular biology to biochemistry and biophysics. Currently we have three main research themes:
1. Mechanisms of folding and assembly of newly synthesized proteins.
Cells from bacteria to humans have evolved a multilayered machinery that engages translating ribosomes to promote folding and assembly of newly synthesized proteins. Using ribosome profiling, genetics and protein biochemistry, we want to understand how this machinery guides nascent polypeptides to the native state, and how assembly of oligomeric protein complexes is achieved in pro- and eukaryotes.
2. Mechanisms of protein quality control.
Disrupting proteostasis of living cells activates protective quality control systems, which refold or degrade misfolded proteins or sequester potentially cytotoxic misfolded proteins into aggregates, deposited at specific intracellular sites. We want to understand the cellular processes leading to targeted deposition of aggregating proteins inside cells. We are also dissecting the mechanisms by which the Hsp70 chaperone network and the AAA+ disaggregase Hsp104 solubilize and refold aggregated proteins, including disease-associated amyloid fibrils.
3. Propagation of protein misfolding in neurodegenerative diseases (Project group Carmen Nussbaum-Krammer).
Neurodegenerative diseases exhibit a complex pathology involving non-cell autonomous effects and progressive spreading of protein misfolding. Using the metazoan model system C . elegans we want to understand how local protein misfolding is affecting neighboring cells and tissues and how proteostasis is orchestrated at the organismal level.
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Journal of molecular biologypp.168484-168484, (2024)
Melania Minoia, Jany Quintana-Cordero, Katharina Jetzinger, Ilgin Eser Kotan,Kathryn Jane Turnbull,Michela Ciccarelli,Anna E. Masser, Dorina Liebers, Eloïse Gouarin, Marius Czech,Vasili Hauryliuk,Bernd Bukau,
Nature Communicationsno. 1 (2024): 1-16
bioRxiv (Cold Spring Harbor Laboratory) (2023)
Jaroslaw Marszalek, Paolo De Los Rio,Douglas Cyr,Matthias P. Mayer, Vasista Adupa,Claes Andréasson, Gregory L. Blatch,Janice Braun,Jeffrey L. Brodsky,Bernd Bukau,J. Paul Chapple, Charlotte Conz,
Joseph George Beton, Jim Monistrol,Anne Wentink, Erin C Johnston,Anthony John Roberts,Bernd Gerhard Bukau,Bart W Hoogenboom,Helen R Saibil
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