Dianthus sylvestris subsp. sylvestris as a promising candidate for phytostabilization of copper-contaminated post-mining sites in Alpine ecosystems

The lack of remediation of inactive mine sites is a serious global concern, as they pose risks to the environment, human health, and safety. The potential of Dianthus sylvestris subsp. sylvestris to remediate post-mining sites contaminated with copper (Cu) at high altitudes, which is a challenging task for most management strategies, was explored in this study. More than 1300 mg Cu kg(-1) in shoots were found in plants collected at the Monte Avanza legacy mine site (Alps). However, it is unclear whether this is due to hyperaccumulation or foliar contamination. To address this gap, field samples were washed with two different protocols, and a controlled Cu-tolerance test was conducted. While very high Cu concentrations, exceeding the Cu hyperaccumulation threshold of 300 mg kg(-1), were found in samples washed with water, results for the plants cleaned with a more rigorous approach with EDTA suggested Cu exclusion strategy. Under controlled conditions, the plant showed Cu hypertolerance but did not hyperaccumulate Cu. Thus D. sylvestris presents a Cu exclusion strategy rather than hyperaccumulation potential, making it a suitable candidate for Cu phytostabilization at high altitude legacy mine sites. The study emphasizes the need for experiments under controlled conditions when assessing the phytoremediation potential.